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Vitamin D, Total (25[OH])


Utilizes a two-step competitive binding immunoenzymatic assay.

Test Utility

Used for the quantitative determination of total 25-hydroxyvitamin D levels in human serum and plasma. Vitamin D aids in controlling the amount of calcium and phosphate levels in the body.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • Lithium Heparin (green top)

Specimen Storage and Stability

Store serum or plasma at the following temperatures: 

  • Room temperature: 15°C to 30°C no longer than 72 hours 

  • Refrigerated: 2°C to 10°C up to 1 week 

  • Frozen: -20°C or lower up to year

Specimen Rejection

  • Broken or leaking tube

  • Insufficient sample volume

  • Frozen whole blood

  • Specimen hemolyzed

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal


Vibra Health Laboratory 

1307- A, Allen Dr 

Troy, MI 48083 

(248) 846-0663

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