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Protein, Total


The Total Protein assay is a modification of the Weichselbaum method, where a violet-colored complex is produced when cupric ions react with proteins and polypeptides in an alkaline solution.  The absorbance of this complex at 540/660 nm is directly proportional to the protein concentration of the sample.

Test Utility

This assay detects the sum of all circulation protein in the bloodstream. Total protein values are used in the diagnosis and treatment of many metabolic and nutritional disorders.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Preferred Specimen: One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)

  • Also acceptable: One Green-top Heparin tube. (Minimum Whole Blood: 3.0 mL)

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room temperature: 15°C to 30°C up to 7 days

  • Refrigerated: 2°C to 8°C up to 1 month

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal


Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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