PCP (Phencyclidine) Screen
Immunoassay screen for qualitative detection of PCP in urine.
Phencyclidine (PCP) was originally made available as a surgical anesthetic agent, but it was removed from clinical use due to undesirable side effects both during surgery and in recovery. The illicit use of the drug produces clinical symptoms ranging from confusion, disorientation, stupor, coma, and death in cases of overdose. The PCP screening assay is based on the competition of a drug labeled with enzyme, glucose-6- phosphate dehydrogenase (G6PDH), and the drug from the urine sample for a fixed amount of specific antibody binding sites.
In the absence of the drug from the sample, the specific antibody binds the drug labeled with G6PDH and the enzyme activity is inhibited. This phenomenon creates a direct relationship between the drug concentration in urine and the enzyme activity. The G6PDH activity is determined spectrophotometrically at 340 nm by measuring its ability to convert nicotinamide adenine dinucleotide (NAD) to NADH.
This test is used to detect the presence of illicit or prescription medications in urine.
Specimen Collection and Preparation
At least 1 mL of urine should be collected in a urine specimen container.
Specimen Storage and Stability
Urine specimen should be stored under refrigerated conditions (2–8°C) until analysis. Samples will also be stored under refrigerated conditions on site for 2 weeks post analysis.
Insufficient sample volume
Specimen labeled incorrectly or not labeled
Specimen validity tests indicate adulterated test
Monday – Saturday
Result available within 24-48 hours of receipt in laboratory
NOTE: Specimens are refrigerated (2-8°) for 14 days before disposal
Vibra Health Laboratory
1307- A, Allen Dr
Troy, MI 48083