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Parathyroid Hormone, Intact (Intact PTH)

Methodology

This is a two-site immunoenzymatic (“sandwich”) assay where the final product is read by a luminometer that measures the amount of light generated by the reaction. The light produced is directly proportional to the concentration of PTH in the sample.

Test Utility

This test measures the amount of circulating PTH in blood. PTH is responsible for managing calcium levels and therefore testing PTH levels may be helpful in determining the reason for increased or decreased calcium.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • EDTA (lavender top) OR Heparin (green top)

Specimen Storage and Stability

Store serum at the following temperatures:

  • Room temperature: 15°C to 30°C for no longer than 4 hours

  • Refrigerated: 2°C to 8°C for no longer than 8 hours

  • Frozen: -20°C or lower for no longer than 6 months. Thaw samples no more than 3 times.


Store plasma at the following temperatures:

  • Room temperature: 15°C to 30°C for no longer than 8 hours

  • Refrigerated: 2°C to 8°C for up to 48 hours

  • Frozen: -20°C or lower for no longer than 6 months. Thaw samples no more than 3 times.

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages

Performed

Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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