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Oxycodone Screen


Immunoassay screen for qualitative detection of oxycodone in urine.

Oxycodone is a semi-synthetic opioid prescribed for pain management in patients with moderate to severe pain. It is like codeine and morphine in its analgesic properties but it is more potent than morphine and has higher dependence potential. The drug oxycodone is supplied as OxyContin® (Oxycodone HCl) or in combination with aspirin (Percodan®) or acetaminophen (Percocet®). Drug abusers crush the pills into powder and snort them for faster effect which may result in a potentially fatal outcome. 

Oxymorphone, noroxycodone and noroxymorphone are the only known metabolites of oxycodone. The metabolite, oxymorphone, is a potent narcotic analgesic, while the other two metabolites are relatively inactive. From 33-61% of a single dose of oxycodone is excreted in urine within 24 hours as unconjugated oxycodone (13-19%), conjugated oxycodone (7-29%), and conjugated oxymorphone (13-14%). 

The oxycodone screening assay uses specific antibodies that can detect oxycodone and oxymorphone without any significant cross-reactivity to other opiate compounds. The assay is based on competition between a drug labeled with glucose-6-phosphate dehydrogenase (G6PDH), and free drug from the urine sample for a fixed amount of specific antibody binding sites.

In the absence of free drug from the sample, the specific antibody binds the drug labeled with G6PDH and causes a decrease in enzyme activity. This phenomenon creates a direct relationship between the drug concentration in urine and enzyme activity. The enzyme activity is determined spectrophotometrically at 340 nm by measuring the conversion of nicotinamide adenine dinucleotide (NAD) to NADH.

Test Utility

This test is used to detect the presence of illicit or prescription medications in urine.

Specimen Collection and Preparation

  • At least 1 mL of urine should be collected in a urine specimen container.

Specimen Storage and Stability

Urine specimen should be stored under refrigerated conditions (2–8°C) until analysis. Samples will also be stored under refrigerated conditions on site for 2 weeks post analysis.

Specimen Rejection

  • Insufficient sample volume

  • Specimen labeled incorrectly or not labeled

  • Specimen validity tests indicate adulterated test

Reference Pages


Monday – Saturday

Result available within 24-48 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 14 days before disposal


Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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