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LDL-Cholesterol (Direct Measurement)

Methodology

The LDL-Cholesterol test is a two-reagent homogenous system to determine the amount of LDL-cholesterol in the body.

Test Utility

This test is used to measure the amount of LDL cholesterol or low-density lipoprotein (bad) cholesterol in the blood. It is typically used in conjunction with other laboratory tests such as total cholesterol, HDL cholesterol and triglycerides as an indicator of risk for heart disease. High LDL levels may increase the overall risk for heart disease.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • EDTA (lavender top)

  • Heparin (green top)

Specimen Storage and Stability

Sample should be centrifuged at 3000 rpm for 15 minutes within 3 hours of collection, and serum or plasma separated as soon as possible.


Store serum or plasma at the following temperatures:

  • Refrigerated: 2°C to 8°C up to 5 days

  • Frozen: -70°C or lower up to 3 months. Samples should be thawed only once.

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

  • Citrate and oxalate anticoagulants

Reference Pages

Performed

Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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