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Influenza A/B Assay

Methodology

Isothermal Reverse Transcriptase - Helicase-Dependent Amplification (HDA) in the presence of target-specific fluorescence probes.

Test Utility

Used to detect Influenza A and Influenza B viral RNA in nasopharyngeal swabs from patients with signs and symptoms of respiratory infection.

Specimen Collection and Preparation

Nasopharyngeal Swab - specialized swab with longer, flexible shaft and mini-tip (flocked, foam or spun synthetic with aluminum shaft) in the following tubes (1-3 mL): 

  • Copan eSwab 

  • UTM 

  • M4/M4RT/M5/M6 viral transport media

Specimen Storage and Stability

Store the swab at the following temperatures: 

  • Room temperature: Not indicated 

  • Refrigerated: 2°C to 8°C up to 9 days (UTM/M4/M4RT/M5/M6) 

  • Refrigerated: 2°C to 8°C up to 48 hours (eSwab)

Specimen Rejection

  • Received > 9 days after collection (UTM/M4/M4RT/M5/M6) or > 48 hours (eSwab) 

  • Not transported at 2°C to 8°C 

  • Swab missing 

  • < 50µL volume 

  • Broken or leaking tube 

  • Visible contamination present

  • Specimen labeled incorrectly or not labeled 

Reference Pages

Performed

Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory  

1307- A, Allen Dr 

Troy, MI 48083 

(248) 846-0663

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