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Infectious Mononucleosis (MONOSPOT)

Methodology

MONOSPOT Latex is a one-step rapid latex particle agglutination test.

Test Utility

This test is a qualiltative and semiquantitative assay to detect infectious mononucleosis heterophile antibodies. It is used as an aid in the diagnosis of infectious mononucleosis, which is caused by the Epstein-Barr virus (human gammaherpesvirus 4).

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • EDTA (lavender)

Specimen Storage and Stability

Separate serum and plasma within 4 hours and store samples at the following temperatures:

  • Room Temperature: Not indicated

  • Refrigerated: 2°C to 8°C for up to 48 hours for serum and 24 hours for plasma

  • Frozen: -20°C or lower for serum. Frozen plasma is not acceptable

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood or plasma

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages

Performed

Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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