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HIV Ag/Ab Combo (CHIV) Assay


The HIV assay is a 2-wash antigen/antibody sandwich immunoassay, where antigens in the patient sample are bridged by antibodies also present in the patient sample. The p24 antigen in the sample is bridged by antibody that is in the reagents.

Test Utility

This test is used to determine if a person has been infected with the Human Immunodeficiency virus (HIV). This test tells immune status and not when the patient was infected or if the infection is current.

The test is able to detect HIV p24 antigen and antibodies to HIV Type 1, including Group O (HIV-1 + “O”) and/or HIV Type 2 (HIV-2)

Specimen Collection and Preparation

Serum or plasma samples in the following tubes:

  • Serum separator tube (gold or tiger top)

  • Heparin (green top)

  • EDTA (lavender top)

Specimen Storage and Stability

Separate serum and plasma within 24 hours and store samples at the following temperatures:

  • Room temperature: 15°C to 30°C up to 24 hours

  • Refrigerated: 2°C to 8°C up to 14 days

  • Frozen: -20°C or lower for up to 8 months. Samples should be thawed no more than 3 times.

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal


Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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