top of page
Hepatitis B Surface Antigen (HBsAg & Reflex Confirmation)


The HBsAg assay is a sandwich immunoassay that uses direct chemiluminometric technology.

Test Utility

This test is used to determine recent or long-standing infection from the hepatitis B virus (HBV) through detection of antibodies on the surface of the virus.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • EDTA (lavender top) and Lithium/Sodium Heparin (green top)

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room temperature: Up to 8 hours

  • Refrigerated: 2°C to 8°C for up to 14 days

       2°C to 8°C for up to 7 days (Primary tube samples – stoppered and upright)

Primary samples include serum on the clot, plasma on packed red cells andgel barrier collection tubes.

  • Frozen: -20°C or lower. Samples should not be thawed more than 6 times.

Thoroughly mix and centrifuge thawed samples for 2 minutes before using.

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal


Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

bottom of page