Hepatitis B Surface Antigen (HBsAg & Reflex Confirmation)
The HBsAg assay is a sandwich immunoassay that uses direct chemiluminometric technology.
This test is used to determine recent or long-standing infection from the hepatitis B virus (HBV) through detection of antibodies on the surface of the virus.
Specimen Collection and Preparation
Serum or plasma sample in the following tubes:
Serum separator tube (gold or tiger top)
EDTA (lavender top) and Lithium/Sodium Heparin (green top)
Specimen Storage and Stability
Store serum or plasma at the following temperatures:
Room temperature: Up to 8 hours
Refrigerated: 2°C to 8°C for up to 14 days
2°C to 8°C for up to 7 days (Primary tube samples – stoppered and upright)
Primary samples include serum on the clot, plasma on packed red cells andgel barrier collection tubes.
Frozen: -20°C or lower. Samples should not be thawed more than 6 times.
Thoroughly mix and centrifuge thawed samples for 2 minutes before using.
Insufficient sample volume
Sample not stored at proper temperature
Frozen whole blood
Severely hemolyzed samples
Severely lipemic specimens
Specimen labeled incorrectly or not labeled
Monday – Saturday
Result available within 24 hours of receipt in laboratory
NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal
Vibra Health Laboratory
1307- A, Allen Dr
Troy, MI 48083