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Hepatitis B Core Antigen (Total Antibodies)

Methodology

The HBc Total assay is a two-wash antigen immunoassay where antigens are bridged by antibody present in the sample. Results above the assay cut-off are not indicative of antibody level.

Test Utility

The HBc total antibodies test detects both IgM and IgG antibodies. This test may be used to determine infections status in the “window Period” between acute infection and long-term infection.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • EDTA (lavender top) and Lithium/Sodium Heparin (green top)

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room temperature: Up to 12 hours

  • Refrigerated: 2°C to 8°C for up to 3 days

  • Frozen: -20°C or lower. Samples should not be thawed more than 2 times.


Thoroughly mix and centrifuge thawed samples for 2 minutes before using.

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages

Performed

Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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