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Hepatitis A Virus Antibody Total (HAV Total)

Methodology

The HAV Total assay is a competitive immunoassay that uses direct chemiluminescent technology.

Test Utility

This assay detects all antibodies against HAV to determine if the patient has a current or past infection. It is often used to determine if the patient has been vaccinated or used in conjunction with other tests to verify a current or past infection.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • EDTA (lavender top) and Lithium/Sodium Heparin (green top)

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room temperature: Up to 12 hours

  • Refrigerated: 2°C to 8°C for up to 24 hours (Primary tube samples – stoppered and upright)


Primary samples include serum on the clot, plasma on packed red cells gel barrier collection tubes


2°C to 8°C for up to 7 days (Secondary tube samples– stoppered and upright)

  • Frozen: -20°C or lower for up to 1 year. Samples should not be thawed more than 4 times.


Thoroughly mix and centrifuge thawed samples for 2 minutes before using. Supernatant should be collected in a clean vial.

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages

Performed

Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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