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Hemoglobin and Hematocrit (HGB and HCT)


HGB and HCT is performed on the Sysmex XN Series Hematology Analyzer. This analyzer uses cumulative pulse height detection to determine HCT. HGB is converted to SLS- hemoglobin and read photometrically.

Test Utility

This test provides information about hemoglobin and hematocrit in a blood sample. It is often used in the identification of hematological disorders such as anemia, leukemia, inflammatory disease, as well as other disorders.

Specimen Collection and Preparation

Whole blood sample in the following tubes:

  • Preferred Specimen: Lavender Top K2 EDTA tube. (Minimum Whole Blood: 1.5 mL)

  • Also acceptable: Light blue top sodium citrate tube (Minimum Whole Blood: 1.5 mL)

Specimen Storage and Stability

Store whole blood at the following temperatures:

  • Room temperature: 24 hours (<8 hours ideal)

  • Refrigerated 2°C to 8°C:  48 hours

  • Frozen: Do not freeze

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal


Vibra Health Laboratory  

1307- A, Allen Dr 

Troy, MI 48083 

(248) 846-0663

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