Hemoglobin and Hematocrit (HGB and HCT)
Methodology
HGB and HCT is performed on the Sysmex XN Series Hematology Analyzer. This analyzer uses cumulative pulse height detection to determine HCT. HGB is converted to SLS- hemoglobin and read photometrically.
Test Utility
This test provides information about hemoglobin and hematocrit in a blood sample. It is often used in the identification of hematological disorders such as anemia, leukemia, inflammatory disease, as well as other disorders.
Specimen Collection and Preparation
Whole blood sample in the following tubes:
Preferred Specimen: Lavender Top K2 EDTA tube. (Minimum Whole Blood: 1.5 mL)
Also acceptable: Light blue top sodium citrate tube (Minimum Whole Blood: 1.5 mL)
Specimen Storage and Stability
Store whole blood at the following temperatures:
Room temperature: 24 hours (<8 hours ideal)
Refrigerated 2°C to 8°C: 48 hours
Frozen: Do not freeze
Specimen Rejection
Insufficient sample volume
Sample not stored at proper temperature
Frozen whole blood
Hemolyzed samples
Severely lipemic specimens
Specimen labeled incorrectly or not labeled
Reference Pages
Performed
Monday – Saturday
Result available within 24 hours of receipt in laboratory
NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal
Contacts
Vibra Health Laboratory
1307- A, Allen Dr
Troy, MI 48083
(248) 846-0663