ETG (Alcohol) Screen
Immunoassay screen for qualitative detection of alcohol in urine.
Ethyl Glucuronide (ETG) is a direct metabolite of ethanol, formed by enzymatic conjugation of ethanol with glucuronic acid. Alcohol in urine is usually detected for only a few hours, whereas ETG can be detected for up to several days after complete elimination of alcohol from the body. The DRI ETG screen in an enzyme immunoassay.
The assay uses specific antibodies that can detect Ethyl Glucuronide without any significant cross-reactivity to other glucuronide compounds. The assay is based on competition between a drug labeled with glucose-6-phosphate dehydrogenase (G6PDH), and free drug from the urine sample for a fixed amount of specific antibody binding sites. In the absence of free drug from the sample, the specific antibody binds the drug labeled with G6PDH and causes a decrease in enzyme activity. This phenomenon creates a direct relationship between the drug concentration in urine and enzyme activity. Active enzyme converts NAD to NADH resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.
This test is used to detect the presence of illicit or prescription medications in urine.
Specimen Collection and Preparation
At least 1 mL of urine should be collected in a urine specimen container.
Specimen Storage and Stability
Urine specimen should be stored under refrigerated conditions (2–8°C) until analysis. Samples will also be stored under refrigerated conditions on site for 2 weeks post analysis.
Insufficient sample volume
Specimen labeled incorrectly or not labeled
Specimen validity tests indicate adulterated test
Monday – Saturday
Result available within 24-48 hours of receipt in laboratory
NOTE: Specimens are refrigerated (2-8°) for 14 days before disposal
Vibra Health Laboratory
1307- A, Allen Dr
Troy, MI 48083