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D-Dimer

Methodology

This test is performed on a Beckman AU fully automated system. Immune complexes are formed in solution that scatter light in proportion to their size, shape and concentration. Turbidimeters within the system measure the reduction of incident light due to the formation of these immune complexes.

Test Utility

Elevated levels of D-Dimer can occur in several conditions associated with fibrin breadown such as Deep Vein Thrombosis (DV), Pulmonary Embolism (PE) and Disseminated Intravascular Coagulation (DIC).

Specimen Collection and Preparation

Collect a blood sample in the following tube:

  • Light Blue 3.2% Sodium Citrate Tube – Tube must be filled to 90% of minimum/maximum etched fill line

  • If possible, centrifuge the specimen at 3000 rpm for 15 min, remove plasma and freeze

Specimen Storage and Stability

Store whole blood at the following temperatures:

  • Room temperature: 4 hours

  • Refrigerated 2°C to 8°C:  Separated plasma only. Up to 4 days

  • Frozen at -20°C: Separated plasma only. Up to 6 months

Specimen Rejection

  • Insufficient sample volume – Tube must be filled to with 90% of etched fill line

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages

Performed

Monday – Sunday, 16 hours a day 

Result available within 24 hours of receipt in laboratory 

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory  

1307- A, Allen Dr Troy, MI 48083 

(248) 846-0663

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