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Creatine Kinase (CK)


CK reversibly catalyzes the transfer of a phosphate group to produce ATP, which is used to produce glucose-6-phosphate from glucose followed by oxidization to G6-DH, which is further reduced to NADP. Ultimately NADPH is formed and is proportional to the CK activity in the patient sample. NADPH is detected by an increase in absorbance.

Test Utility

The enzyme Creatine kinase (CK) is found in skeletal muscle, heart muscle and brain. Damage to these tissues results in an increase of creatine kinase in the bloodstream. Elevated CK levels may indicate muscle injury or disease.

Note: This tests for total creatine kinase. See Creatine Kinase, Isoenzyme MB if the CK-MB isoform is requested. 

Specimen Collection and Preparation

Protect samples from light for maximum stability

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • Lithium/Sodium Heparin (green top)

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room temperature: Up to 4 hours

  • Refrigerated: 2°C to 8°C for up to 12 hours

  • Frozen: -20°C or lower for up to 1 month.

Specimen Rejection

  • Insufficient sample volume

  • EDTA OR Oxalate OR Citrate plasma

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal


Vibra Health Laboratory  

1307- A, Allen Dr 

Troy, MI 48083 

(248) 846-0663

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