Creatine Kinase, Isoenzyme MB (CK-MB)
Note: This test is for the CK-MB isoform. If a total CK level is desired, see Creatine Kinase (CK).
The CK-MB assay is a two-site immunoenzymatic (“sandwich”) assay that utilizes mouse monoclonal anti-human CK-MB antibodies, paramagnetic particles and chemiluminescence for the quantitative determination of CK-MB on the Beckman Access2 Immunoassay System.
Used for the quantitative determination of CK-MB levels in human serum and plasma. CK-MB is one of three isoforms of CK and is expressed in muscle (M) and brain (B). It is often used to determine if muscle damage, especially after an acute mycocardial infarction, has occurred.
Specimen Collection and Preparation
Plasma sample in the following tubes:
Lithium heparin (green top)
Specimen Storage and Stability
Sample should be centrifuged at 3000 rpm for 15 minutes within 1 hour of collection, and plasma separated as soon as possible.
Store plasma at the following temperatures:
Room temperature: 15°C to 30°C no longer than 8 hours
Refrigerated: 2°C to 8°C up to 48 hours
Frozen: -20°C or lower up to 7 days
Specimen received not centrifuged
Sample not stored at proper temperature
Broken or leaking tube
Insufficient sample volume
Clotted or hemolyzed samples
Specimen labeled incorrectly or not labeled
Monday – Sunday, 16 hours a day
Result available within 24 hours of receipt in laboratory
NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal
Vibra Health Laboratory
1307- A, Allen Dr Troy, MI 48083