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Complete Blood Count with Differential (CBC + Diff)

Methodology

The CBC with differential is performed on the Sysmex XN Series Hematology Analyzer. This analyzer uses electronic resistance detection, photometric analysis and flow cytometry with a semiconductor laser to determine whole blood diagnostic parameters.

Test Utility

This test provides information about the RBC (red blood cells), WBC (white blood cells) and platelets in a blood sample. It is often used in the identification of hematological disorders such as anemia, leukemia, inflammatory disease, as well as other disorders. The differential identifies the percentage of each WBC type within the sample.

Specimen Collection and Preparation

Whole blood sample in the following tubes:

  • Preferred Specimen: Lavender Top K2 EDTA tube. (Minimum Whole Blood: 1.5 mL)

  • Also acceptable: Light blue top sodium citrate tube (Minimum Whole Blood: 1.5 mL)

Specimen Storage and Stability

Store whole blood at the following temperatures:

  • Room temperature: 24 hours (<8 hours ideal)

  • Refrigerated 2°C to 8°C:  48 hours

  • Frozen: Do not freeze

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages

Performed

Monday – Sunday, 16 hours a day 

Result available within 24 hours of receipt in laboratory 

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory  

1307- A, Allen Dr Troy, MI 48083 

(248) 846-0663

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