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CO² (Bicarbonate)

Methodology

This procedure uses the method developed by Forrester et al., that utilizes an enzymatic reaction.

Test Utility

Used to evaluate the carbonate buffering system that determines acid-base balance. High results may indicate respiratory acidosis with CO2 retention, and low results may indicate respiratory alkalosis.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Preferred Specimen: One Gold-top or red top SST tube. (Minimum Whole Blood: 4.0 mL)*

  • Also acceptable: One Lithium Heparin or Sodium Heparin tube (Minimum Whole Blood: 3.0 mL)*

  • *Specimen should be tightly stoppered

Specimen Storage and Stability

Serum Specimens:

  • Room Temperature (20–25°C F): 24 hours

  • Refrigerated (2–8°C): Not indicated

  • Frozen (-20°C or below): Not indicated

Plasma Specimens:

  • Room Temperature (20–25°C F): 24 hours

  • Refrigerated (2–8°C): Not indicated

  • Frozen (-20°C or below): Not indicated

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages

Performed

Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

STAT results available within 2 hours of receipt in the laboratory

Contacts

Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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