Calcium Ions (Ca2+) react with Arsenazo III to form a purple complex. The absorbance of the Ca-Arsenazo III complex is measured biochromatically at 660/700 nm. The increase in absorbance of the reaction mixture is directly proportional to the calcium concentration in the sample.
Calcium measurement aids in the diagnosis and management of several diseases involving the parathyroid glands, pancreas, kidneys, gastrointestinal tract, bones and endocrine system.
Specimen Collection and Preparation
Sample should be centrifuged at 3000 rpm for 15 minutes within 1 hour of collection, and plasma separated as soon as possible.
Serum or plasma sample in the following tubes:
Serum separator tube (gold or tiger top)
Lithium Heparin (green top)
Specimen Storage and Stability
Store serum or plasma at the following temperatures:
Room temperature: 15°C to 30°C up to 7 days
Refrigerated: 2°C to 8°C up to 22 days
Frozen: -20°C or lower up to 1 year
Insufficient sample volume
Sample not stored at proper temperature
Frozen whole blood
Severely lipemic specimens
Specimen labeled incorrectly or not labeled
Monday – Saturday
Result available within 24 hours of receipt in laboratory
NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal
Vibra Health Laboratory
1307- A, Allen Dr
Troy, MI 48083