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Bilirubin, Total


A stabilized diazonium salt, 3,5-dichlorophenyldiazonium tetrafluoroborate (DPD), reacts with bilirubin to form azobilirubin which absorbs at 570/660 nm.

Test Utility

An indication of hepatic function and biliary disease, including hepatitis, cirrhosis, and other advanced neoplastic diseases involving the liver.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Preferred Specimen: One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)*

  • Also acceptable: One Lavender-top EDTA tube. (Minimum Whole Blood: 3.0 mL)*

  • Also Acceptable: One Green top Lithium Heparin tube. (Minimum Whole Blood: 4.0 mL)*

*Protect from direct light as soon as possible

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room Temperature: Not indicated

  • Refrigerated: 2°C to 8°C up to 3 days*

  • Frozen: -20°C or lower up to 3 months*

*Protect sample from light

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Sample that has been excessively exposed to light.

  • Frozen whole blood

  • Hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

STAT results available within 2 hours of receipt in the laboratory


Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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