top of page
Benzodiazepine Screen


Immunoassay screen for qualitative detection of benzodiazepine in urine.

Benzodiazepines are sedative-hypnotic drugs, which are subject to abuse. Benzodiazepines are structurally similar and include a wide variety of drugs such as alprazolam, chlordiazepoxide, diazepam, lorazepam, oxazepam and triazolam. They are absorbed and metabolized at different rates, resulting in various psychoactive effects. Therefore, the detection of benzodiazepines or their metabolites in urine can be used as an indicator of benzodiazepine abuse.

The benzodiazepine screening assay uses a specific antibody which can detect most benzodiazepines and their metabolites in urine. The assay is based on the competition of an enzyme glucose6-phosphate dehydrogenase (G6PDH) labeled drug and the drug from the urine sample for a fixed amount of specific antibody binding sites. In the absence of free drug from the sample, the enzyme-labeled drug is bound by the specific antibody and the enzyme activity is inhibited. This phenomenon creates a relationship between drug concentration in urine and enzyme activity. The enzyme G6PDH activity is determined spectrophotometrically at 340 nm by measuring its ability to convert nicotinamide adenine dinucleotide (NAD) to NADH.

Test Utility

This test is used to detect the presence of illicit or prescription medications in urine.

Specimen Collection and Preparation

At least 1 mL of urine should be collected in a urine specimen container.

Specimen Storage and Stability

Urine specimen should be stored under refrigerated conditions (2–8°C) until analysis. Samples will also be stored under refrigerated conditions on site for 2 weeks post analysis.

Specimen Rejection

  • Insufficient sample volume

  • Specimen labeled incorrectly or not labeled

  • Specimen validity tests indicate adulterated test

Reference Pages


Monday – Saturday

Result available within 24-48 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 14 days before disposal


Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

bottom of page