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Aspartate Aminotransferase (AST or SGOT)


Measures the conversion rate of aspartate and α-oxoglutarate to L-glutamate and oxalacetate by aspartate aminotransferase. Oxalacetate is subsequently reduced to L-malate with NADH conversion to NAD+. The consumption of NADH is measured in this assay and is an indicator of AST activity.

Test Utility

For the quantitative determination of aspartate aminotranserase activity in human serum. It is commonly used to determine liver damage.

Specimen Collection and Preparation

Serum or heparinized plasma sample in the following tubes:

  • Preferred Specimen: One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)

  • Also acceptable: One Lithium Heparin tube (Minimum Whole Blood: 3.0 mL)

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room temperature: 15°C to 30°C no longer than 24 hours

  • Refrigerated: 2°C to 8°C up to 4 weeks

  • Frozen: -20°C or lower up to 1 year

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Sunday, 16 hours a day 

Result available within 24 hours of receipt in laboratory 

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal


Vibra Health Laboratory  

1307- A, Allen Dr Troy, MI 48083 

(248) 846-0663

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