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Anti-HBsAg (Antibodies to Hepatitis B Surface Antigen)


The Anti-HBsAg assay is a sandwich immunoassay that uses direct chemiluminescent technology.

Test Utility

The Anti-HBsAg test is used to determine immunity to HBV through either viral infection or vaccination against HBV.

Specimen Collection and Preparation

Serum or plasma sample in the following tubes:

  • Serum separator tube (gold or tiger top)

  • EDTA (lavender top) and Lithium/Sodium Heparin (green top)

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room temperature:

  • Up to 7 days – Serum and EDTA plasma

  • Up to 3 days – Heparin plasma

  • Refrigerated 2°C to 8°C:

  • Up to 7 days (Primary tube samples – stoppered and upright)

  • Frozen: -20°C or lower

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Severely hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages


Monday – Saturday

Result available within 24 hours of receipt in laboratory

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal


Vibra Health Laboratory

1307- A, Allen Dr

Troy, MI 48083

(248) 846-0663

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