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Alkaline Phosphatase (ALP)

Methodology

Measures the conversion rate of p-nitro-phenylphosphate (pNPP) to para-nitrophenol (pNP) by alkaline phosphatase, which is directly proportional to ALP activity in the sample.

Test Utility

Used in the diagnosis of hepatobiliary disorders and bone disease

Specimen Collection and Preparation

Serum or heparinized plasma sample in the following tubes:

  • Preferred Specimen: One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)

  • Also acceptable: One Lithium Heparin tube (Minimum Whole Blood: 3.0 mL)

Specimen Storage and Stability

Store serum or plasma at the following temperatures:

  • Room temperature: Not indicated

  • Refrigerated: 2°C to 8°C up to 4 days

  • Frozen: -20°C or lower up to 30 days

Specimen Rejection

  • Insufficient sample volume

  • Sample not stored at proper temperature

  • Frozen whole blood

  • Hemolyzed samples

  • Severely lipemic specimens

  • Specimen labeled incorrectly or not labeled

Reference Pages

Performed

Monday – Sunday, 16 hours a day 

Result available within 24 hours of receipt in laboratory 

NOTE: Specimens are refrigerated (2-8°) for 7 days before disposal

Contacts

Vibra Health Laboratory 

1307- A, Allen Dr Troy, MI 48083 

(248) 846-0663

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